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In the present research, immunohistological and immunoblot analyses were used to examine the expression off
the neural cell adheion molecule (N-CAM) and the neuron-glia
cell adhesion molecule (Ng-CAM) within totally different parts of a functionally linkedd neuromuscular
system extending from skeetal muscle to the spinal cord after peripheral nnerve harm.
Based on the rationale that such molecules include a sign sequence
with a view tto be presented on the cell floor, now wee have employed
a eukaryotic-based mostly, signal-sequence-entice strategy to identify novel secreted and membrane-bound molecules thhat are expressed in myelinating and non-myelinating Schwann cells.
Our results indicate that myelinating Schwann cells specific a bqttery
of novel CAMs which may mediate their interactions with the underlying axons.
Using cDNA libraries derived from dbcAMP-stimulated primary Schwann
cells aand 3-day-previous rat sciatic nerve mRNAs, we generated an intensive listing of novel
molecules expressed in myelinating nerves within the PNS.
Anti-N-CAM antibkdies stained neurites, Schwann cells, and
the perineurium of the regenerating sciatic nerve.
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